Medium power: DQ stain
Image showing malignant cells in sheets with enlarged irregular nuclei, prominent nucleoli and wispy delicate cytoplasm.
Medium power: DQ stain
Image showing cohesive 3D clusters of malignant cells with enlarged irregular nuclei and wispy delicate cytoplasm.
High power: Pap stain
Image showing malignant cells with enlarged nuclei, prominent nucleoli and wispy delicate cytoplasm.
High power: Pap stain
Image showing malignant cells with enlarged irregular nuclei with pleomorphism, prominent nucleoli, coarse chromatin and wispy delicate cytoplasm
H&E stained cell block section showing malignant cells with enlarged irregular nuclei, prominent nucleoli, and occasional intracytoplasmic mucin.
H&E stained cell block section showing malignant cells with enlarged irregular nuclei, macronucleoli, and occasional intracytoplasmic mucin.
Features
• Flat sheets, acini, 3D clusters, papillary, single cells
• Delicate and finely vacuolated cytoplasm
• Intranuclear cytoplasmic inclusions
• Intracytoplasmic lumens with mucin
• Psammoma bodies (papillary subtype)
• Enlarged and irregular
• Prominent nucleoli, often cherry red macronucleoli
• Open chromatin
• Mucinous, non-mucinous, papillary, clear cell, colloid, hepatoid
• Positive for cytokeratin, CK7, TTF-1 (may be negative in mucinous), NapsinA; negative for CK20 (may be positive in mucinous), CDX2
• May have mutations in EGFR, KRAS, BRAF; rearrangements in ALK, ROS1
Auger M et al. Diagn Cytopathol. 1997 Mar;16(3):253-7.
Atkins KA. Am J Clin Pathol. 2004 Jul;122(1):14-6.