High power: DQ stain
Clusters of acinic cells with granular and vacuolated cytoplasm
Low power: Pap stain
Cellular smear with a sheet of acinic cells with round nuclei and granular cytoplasm
High power: Pap stain
Acinic cells with round nuclei, prominent nucleoli, and granular cytoplasm
High power: Pap stain
Acinic cells with round nuclei, prominent nucleoli, and granular cytoplasm.
Note bluish-red zymogen granules in the cytoplasm.
High power: Pap stain
Acinic cells with round nuclei, prominent nucleoli, and granular cytoplasm
Cell block: H&E stain
Acinic cells with round nuclei, visible to prominent nucleoli, and granular to vacuolated cytoplasm. Degranulated cytoplasm results in clear cell change.
Cell block: H&E stain
Acinic cells with round nuclei, visible to prominent nucleoli, and granular to vacuolated cytoplasm.
Medium power: DQ stain
Clusters of acinic cells.
Note granular and vacuolated cytoplasm.
HIgh power: DQ stain
Clusters of acinic cells.
Note granular and vacuolated cytoplasm.
Medium power: Pap stain
Clusters of acinic cells
Medium power: Pap stain
Clusters of acinic cells with round nuclei and granular cytoplasm
Cell block: PAS-D stain
Intracytoplasmic zymogen granules are positive for diastase resistant periodic acid-Schiff (PAS-D) stain
Features
• Solid monotonous sheets, cystic, papillary growth pattern
• Resembles normal acinar cells
• Bunch of grapes appearance
• Cellularity with acinic cells only (no ducts or stroma)
• Acinic cells with granular or vacuolated cytoplasm
• Clear cells with clear cytoplasm (like RCC)
• May have intercalated ductal cells and glandular cells
• Nuclei round and uniform
• May have binucleation
• Naked nuclei may be present
• Fine to coarse chromatin
• May have prominent nucleoli
• May have intranuclear cytoplasmic inclusions
• Mitosis and necrosis typically rare
• May have chronic inflammation
• May have psammoma bodies
• Typically stroma or mucin no present
• Express cytokeratin, zymogen granules PAS positive (PTAH negative D/D oncocytes)
Palma O et al. Acta Cytol. 1985 Jul-Aug;29(4):516-21.
Klijanienko J et al. Diagn Cytopathol. 1997 Nov;17(5):347-52.