Low power: DQ stain
Monotonous small lymphoid cells
Low power: Pap stain
Dispersed small lymphoid cells with scant cytoplasm
High power: DQ stain
Lymphoid cells with irregular nuclear contours
High power: Pap stain
Note irregular nuclear contours with condensed chromatin
Cell block: H&E stain
Small to medium lymphoid cells with irregular nuclear contours and condensed chromatin
Cell block: BCL1 stain (Cyclin D1)
Positive nuclear stain in neoplastic cells
Low power: DQ stain
Blastoid variant: Monotonous population of medium to large cells; frequent mitotic figures
High power: DQ stain
Blastoid variant: Monotonous population of medium to large cells; frequent mitotic figures
Features
• Dispersed monomorphic population
• Lymphoid cells with scant cytoplasm
• Classical: Monotonous population of small to medium cells
• Blastoid variant: Monotonous population of medium to large cells (resemble lymphoblasts)
• Pleomorphic variant: Heterogeneous population of large cells
• Classical: Irregular nuclear contours with condensed chromatin
• Blastoid variant: Dispersed chromatin
• Pleomorphic variant: Oval to irregular nuclear contours; prominent nucleoli
• Frequent mitoses in blastoid and pleomorphic variants
• Typically positive for Pan-B-cell markers, CD5, Cyclin D1
• Negative for CD10, CD23
• Typically express CD45 (bright), CD19, CD20, CD5, FMC-7; kappa or lambda light-chain restriction
• Negative for CD10, CD23, CD200
• t(11;14)(q13;q32)
• Caraway NP, Stewart JM. Mantle cell lymphoma. Diagnostic Pathology Cytopathology. First Edition. DR Mody. Manitoba: Amirsys, 2014. III-4-24-III-4-25.
• Swerdlow SH, Campo E, et al. Mantle cell lymphoma. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Fourth Edition. Steven H. Swerdlow. Lyon: 2008. 229-232.