Medium power: Pap stain
Cohesive honeycomb sheet of small cuboidal cells with bile pigment in the background
Medium power: Pap stain
Cohesive honeycomb flat sheet of small cuboidal cells
Bile pigment in the background
High power: Pap stain
Cohesive honeycomb flat sheet of small cuboidal cells with round to oval nuclei, smooth nuclear membranes and delicate cytoplasm
Medium power: Pap stain
Benign ductal epithelial cells with a group of ducal cells with reactive change. Note crowding and overlapping of cells with hyperchromasia in cells with reactive change.
High power: Pap stain
Benign ductal epithelial cells with some reactive change
Note smooth nuclear membranes and basally oriented nuclei
Features
• Usually seen as bile duct brushings/ washings
• Cytologically similar to ductal epithelial cells of pancreatic duct
• Cohesive flat orderly honeycomb sheets
• Palisading of cells at the edge
• Rare single intact cells
• Uniform cuboidal cells
• Moderate to abundant delicate cytoplasm
• Goblet cells may be present
• Round to oval nuclei
• Smooth nuclear membranes
• Basally oriented nuclei
• Fine chromatin
• May have small nucleoli
• Granular green-gold bile pigment present
• Mitosis/necrosis usually not present
• Usually in response to inflammation, instrumentation, stents, stones
• Crowding and overlapping of cells
• Nuclear enlargement
• Low N:C ratio
• Smooth nuclear membranes
• Prominent nucleoli
• Mitoses frequently seen