Low power: DQ stain
Dispersed polymorphous lymphoid cells
Low power: Pap stain
Polymorphous lymphoid cells
High power: DQ stain
Mixture of monocytoid cells with abundant cytoplasm, plasma cells, and plasmacytoid cells
High power: Pap stain
Mixture of monocytoid cells with abundant cytoplasm, plasma cells, and plasmacytoid cells
Cell block: H&E stain
Polymorphous lymphoid cells with irregular nuclear contours
Features
• Polymorphous plasmacytic, monocytoid and/or centrocytic cells
• Difficult to distinguish from reactive lymph node
• Excess pale to light blue cytoplasm on Diff-Quick
• Slight nuclear membrane irregularity
• Typically positive for Pan-B-cell markers, CD43 (~50%), Bcl-2
• Negative for CD5, CD10, Bcl-6, Cyclin D1
• Typically express CD45 (bright), CD19, CD20; kappa or lambdalight-chain restriction
• Negative for CD5, CD10
• No consistent translocations identified
• Caraway NP, Stewart JM. Nodal marginal zone lymphoma. Diagnostic Pathology Cytopathology. First Edition. DR Mody. Manitoba: Amirsys, 2014. III-4-26-III-4-27. Print.
• Campo E, Pileri SA. et al. Nodal marginal zone lymphoma. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Fourth Edition. Steven H. Swerdlow. Lyon: 2008. 218-219. Print.