Cell block: H&E stain
Clusters of epithelioid cells with large irregular nuclei and prominent nucleoli present within the cell block ,,11,H&E,Cell block
Bone & Soft Tissue,Epithelioid Hemangioendothelioma,01 : BoneSoft Tissue Epithelioid Hemangioendothelioma,http://proqms.info/Images/BoneSoftTissueEpithelioidHemangioendothelioma01.jpg,Low power: Pap Stain
DQ stain: Low power
Hypercellular smear with predominantly singly dispersed cells and small clusters
DQ stain: Medium power
Small clusters and singly dispersed epithelioid cells
DQ stain: Medium power
Small clusters and singly dispersed epithelioid cells with round nuclei and dense cytoplasm
DQ stain: High power
Large epithelioid cells with round to pleomorphic nuclei and bi/multinucleation
Pap stain: Low power
Hypercellular smear with epithelioid cells in small clusters and singly
Pap stain: Medium power
Loosely cohesive clusters of epithelioid cells. Note stromal fragment in the background
Pap stain: High power
Epithelioid cells with enlarged irregular nuclei, multinucleation, coarse chromatin, prominent nucleoli and dense cytoplasm
Pap stain: High power
Epithelioid cells in small clusters with enlarged irregular nuclei, coarse chromatin, and dense cytoplasm
Pap stain: High power
Occasional large anaplastic cells with large nuclei and multinucleation present
Cell block: H&E stain
Clusters of epithelioid cells seen in the cell block
Features
• Commonly affects hand and wrist
• Usually flexor surfaces (hand, forearam, knee, lower leg, ankle)
• Proximal type may be seen in pelvis, peritoneum, genital tract
• Ages 20-40
• Male predilection
• Biphasic epithelioid and spindle cells
• Single cells, cords, nests
• Epithelioid round to polygonal cells
• Dense cytoplasm
• Rhabdoid cytoplasmic inclusions may be present
• Plump spindle cells may be admixed
• Nuclei may be bland to anaplastic
• Multinucleation
• Coarse chromatin
• Prominent nucleoli
• Multinucleated giant tumor cells
• Mitosis present, including atypical mitotic figures
• Necrosis present
• Fibrillary stromal fragments
• IHC: positive for vimentin, cytokeratin, EMA, may have CD34, CD99, calretinin, SMA; negative for CD31; loss of INI1
• Zeppa P et al. Diagn Cytopathol. 1999 Dec;21(6):405-8.
• Cardillo M et al. Cancer. 2001 Aug 25;93(4):246-51.