Low power: Pap stain
Moderate cellularity
Cohesive groups and numerous naked nuclei in background
Medium power: Pap stain
Cohesive group with transgressing small vessels
Note acinar arrangement of cells
High power: Pap stain
Acinar arrangement of cells
Note uniformity of cells and abundant cytoplasm
Medium power: DQ stain
Note the cohesive groups are larger than normal acini
Acinar arrangement, uniform cells and abundant cytoplasm
Medium power: DQ stain
Variability in amount of cytoplasm in two groups
Nuclear cytoplasmic ratio is low despite variability
Note naked nuclei in the background
High power: Pap stain
Acinar arrangement, abundant cytoplasm
Delicate cell membrane
Uniformly round nuclei
High power: Pap stain
Note uniform round nuclei, single prominent nucleolus
Cytoplasm is abundant and granular
High power: DQ stain
Cytoplasm is abundant and granular
Note cytoplasmic vacuoles/frothy appearance
High power: DQ stain
Abundant and frothy cytoplasm in the group
Numerous naked nuclei in background
High power: Pap stain
Hyaline globules present in background
High power: Pap stain
Single group with acinar arrangement, abundant cytoplasm
Note prominent nucleoli
Hyaline globule present in background
Medium power: H&E stain on cell block
Large cohesive group with acinar arrangement
Frothy cytoplasm and uniform round nuclei
High power: Trypsin stain
Note granular cytoplasmic staining
Features
• Moderate cellularity
• Loosely cohesive clusters
• Occasional single cells
• Rare groups with small vessels
• Prominent acinar formation
• Uniform polarized cells (Plasmacytoid)
• Abundant apical cytoplasm
• Pale foamy to eosinophilic granular cytoplasm
• Basal nuclear location
• Round nuclear configuration
• Rare binucleated cells
• Single prominent nucleolus
• Clumped chromatin
• Lack of atypia
• Numerous nuclei devoid of cytoplasm (naked nuclei)
• Occasional cases with hyaline globules
• Rare to absent mitoses
• Rare to absent necrosis
• Express cytokeratin, CK8, CK18, zymogen granules PAS positive; negative for CK7, CK19, CK20
Sigel CS et al. Cancer Cytopathol. 2013 Aug;121(8):459-70.
Stelow EB et al. Diagn Cytopathol. 2006 May;34(5):367-72.