High power: DQ stain
3D clusters of crowded malignant cells with enlarged nuclei, crowding, coarse chromatin, prominent nucleoli, and vacuolated cytoplasm
High power: DQ stain
3D clusters of crowded malignant cells with enlarged nuclei, crowding, coarse chromatin, and prominent nucleoli. Note the abundant vacuolated cytoplasm. This was a clear cell variant.
High power: Pap stain
3D clusters of crowded malignant cells with enlarged nuclei, nuclear irregularity and crowding, coarse chromatin, prominent nucleoli, and vacuolated cytoplasm
Features
• Generally encountered in ascites and peritoneal washing specimens.
• Positive cytology signifies Stage IIIA
• Large and small 3D clusters and single cells
• Scant to moderate cytoplasm
• High N:C ratio
• Enlarged hyperchromatic nuclei
• Pleomorphism seen in high grade tumors
• Irregular nuclear membranes
• Coarse chromatin
• Prominent nucleoli
• Mitosis may be seen
• IHC: Tumor cells typically express Pax8, ER, PR, while negative for calretinin (D/D mesothelial cells) and CD68 or CD163 (histiocytes).
• Ziselman EM et al. Acta Cytol. 1984 Mar-Apr;28(2):105-10.