Low power: DQ stain
Cluster of large cells with small round eccentrically located nuclei and abundant vacuolated cytoplasm.
High power: DQ stain
Cluster of large cells with small round eccentrically located nuclei and abundant vacuolated cytoplasm.
Low power: Pap stain
Clusters and single cells with small round eccentrically located nuclei and abundant vacuolated cytoplasm.
High power: Pap stain
Clusters and sheets of large cells with round to pleomorphic nuclei, irregular nuclear membranes, fine to coarse chromatin, prominent nucleoli and abundant vacuolated cytoplasm. Fuhrman nuclear grade 3.
High power: Pap stain
Clusters of large cells with round enlarged nuclei, irregular nuclear membranes, fine to coarse chromatin, prominent nucleoli and abundant vacuolated cytoplasm.
High power: Pap stain
Clusters and sheets of large cells with round to pleomorphic nuclei, irregular nuclear membranes, fine to coarse chromatin, prominent nucleoli and abundant vacuolated cytoplasm. Fuhrman nuclear grade 3.
Cell block: H&E stain
RCC cells with small round nuclei and more clear cytoplasm (bottom) and higher grade cells with more pleomorphic nuclei and granular cytoplasm (top).
Cell block: H&E stain
RCC cells with small round to irregular nuclei, visible to prominent nucleoli and abundant clear cytoplasm.
Features
• Bloody moderately cellular smears
• Clusters, sheets and single cells
• Large cells
• Abundant vacuolated cytoplasm
• May have areas with granular cytoplasm
• May have metachromatic basement membrane• like material
• Small round to large pleomorphic nuclei
• Eccentrically located nuclei
• Naked stripped nuclei
• Smooth to irregular nuclear membranes
• Fine to coarse chromatin
• Inconspicuous to prominent nucleoli
• May have intranuclear cytoplasmic inclusions
• Hemorrhage
• Necrosis
• May be cystic
• May have calcification
• Grade 1: Round uniform nuclei (10um), inconspicuous nucleoli
• Grade 2: Slightly enlarged irregular nuclei (15um), conspicuous nucleoli (visible at 400x);
• Grade 3: Moderately enlarged irregular nuclei (20um), prominent nucleoli (visible at 100x)
• Grade 4: Markedly enlarged and bizarre nuclei (>20um), macronucleoli (>RBCs)
• Express cytokeratin, EMA, vimentin, CD10, RCC; negative for CK7, CD117
• Loss chromosome 3p, mutation VHL gene
Nguyen GK et al. Clin Lab Med. 1998 Sep;18(3):429-59, vi.
Hidvegi D et al. Acta Cytol. 1979 Nov-Dec;23(6):467-70.