Low power: DQ stain
Cellular smear with crowded clusters of ductal cells
Medium power: DQ stain
Crowded disorganized clusters of ductal cells with hyperchromasia, overlapping nuclei, nuclear irregularity and variation in nuclear size
High power: DQ stain
Crowded disorganized clusters of ductal cells with hyperchromasia, overlapping nuclei, nuclear irregularity and variation in nuclear size
Low power: Pap stain
Crowded disorganized clusters of ductal cells with hyperchromasia, overlapping nuclei, nuclear irregularity and variation in nuclear size
Medium power: Pap stain
Crowded disorganized clusters of ductal cells with hyperchromasia, overlapping nuclei, variation in nuclear size, nuclear irregularity, coarse chromatin and delicate cytoplasm
Note numerous mitotic figures
Medium power: Pap stain
Crowded disorganized clusters of ductal cells with hyperchromasia, overlapping nuclei, variation in nuclear size, nuclear irregularity, and delicate cytoplasm
High power: Pap stain
Well differentiated ductal adenocarcinoma cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin and delicate cytoplasm
Medium power: Pap stain
Crowded 3D ductal cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin, prominent nuclei and delicate cytoplasm
Medium power: Pap stain
Crowded 3D ductal cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin, prominent nuclei (macronucleoli) and delicate cytoplasm
Medium power: Pap stain
Crowded 3D ductal cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin, prominent nuclei (macronucleoli) and delicate cytoplasm
High power: Pap stain
Ductal adenocarcinoma cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin, prominent nuclei (macronucleoli) and delicate cytoplasm
High power: Pap stain
Crowded 3D ductal cells with nuclear enlargement and irregularity, variation in nuclear sizes, coarse chromatin, prominent nuclei (macronucleoli) and delicate cytoplasm
Features
• Moderately to highly cellular smears
• Predominantly ductal cells
• Rarely acinar and islet cells
• Crowded, disorganized groups and single cells
• High N:C ratios
• Delicate cytoplasm
• Intracytoplasmic mucin vacuoles may be seen
• Nuclei enlarged, irregular outlines
• Anisonucleosis
• Hyperchromasia
• Nuclear grooves and foldings
• Fine to coarse chromatin
• Prominent nucleoli, even macronucleoli
• Mitosis
• Necrosis may be seen
• May have pancreatitis
• Express cytokeratin,CK7, CK19, CK8, CK18, CEA, CA19-9, may be positive for MUC1, CK20; usually negative for MUC2
Lin F et al. Cancer. 2003 Feb 25;99(1):44-50
Bardales RH et al. Diagn Cytopathol. 2006 Feb;34(2):140-75.