DQ stain: Low power
Hypercellular smear with small bland uniform cells in clusters and papillary-like arrangement
DQ stain: Medium power
Clusters of cells with round uniform nuclei and abundant vacuolated cytoplasm
DQ stain: High power
Bland uniform cells with round to oval nuclei and abundant vacuolated cytoplasm. Note occasional large cytoplasmic vacuole.
Pap stain: Low power
Hypercellular smear with small bland uniform cells in clusters and papillary-like arrangement
Pap stain: Medium power
Small bland uniform cells in clusters and papillary-like arrangement
Pap stain: High power
Small bland uniform cells with round to oval nuclei, small nucleoli, and abundant vacuolated cytoplasm. Note cytoplasm is granular in some cells.
Pap stain: High power
Small bland uniform cells with round to oval nuclei, small nucleoli, and granular to vacuolated cytoplasm. Note background with granular debris.
Pap stain: High power
Small bland uniform cells in clusters and occasional single cells. Nuclei are round to oval with small nucleoli and granular to vacuolated cytoplasm. Note occasional cell with large intracytoplasmic vacuole.
Pap stain: High power
Small bland uniform cells in clusters and occasional single cells. Nuclei are round to oval with small nucleoli and granular to vacuolated cytoplasm. Note occasional cell with large intracytoplasmic vacuole
Cell block: H&E stain
Clusters of small uniform cells seen in the cell block
IHC: Tumor cells are positive for cytokeratin
IHC: Tumor cells are positive for S100
IHC: Tumor cells are positive for GATA3
Features
• Hypercellular smears
• Sheet-like, papillary, single cells
• May have transgressing vessels through fragments
• Large, bland, round to polygonal cells
• Vacuolated cytoplasm
• May have large vacuole or soap-bubble like multiple vacuoles
• No granules in cytoplasm
• Round to oval, uniform nuclei
• Irregular nuclear membranes
• Naked stripped nuclei may be present
• Open fine chromatin
• Small inconspicuous nucleoli
• Granular debris or cystic
• Histiocytes
• May have mucin
• No matrix-like material
• No necrosis
• No mitosis
• IHC: positive for S100, mammaglobin, GATA-3; negative for DOG-1
• Molecular: defined by t(12;15) (p13;q25) ETV6-NTRK3 gene rearrangement
• Bishop, JA et al. Cancer Cytopathol. 2013 May;121(5):228-33.
• Griffith, CC et al. Cancer Cytopathol. 2013 May;121(5):234-41.