Low power: DQ stain
Intermediate-sized monotonous lymphoid cells
Low power: DQ stain
Intermediate-sized monotonous lymphoid cells with scattered tingible body macrophages
Low power: Pap stain
Intermediate-sized monotonous lymphoid cells
High power: DQ stain
Neoplastic cells with rounded to slightly irregular nuclei and vacuolated cytoplasm.
High power: DQ stain
Neoplastic cells with 1 or multiple nucleoli and a tingible body macrophage (center)
High power: Pap stain
Intermediate-sized monotonous lymphoid cells with rounded to slightly irregular nuclei and 1 or multiple nucleoli
Cell block: H&E stain
Intermediate-sized monotonous lymphoid cells
Cell block: MIB-1 stain
Positive in nearly 100% of the neoplastic cells
Features
• Dispersed cells
• Monomorphic population
• Starry-sky appearance
• Predominantly intermediate-sized cells
• Moderate amount of dense basophilic cytoplasm
• Cytoplasmic vacuoles seen on Diff-Quik-stained smear
• Nuclei round to oval
• Chromatin finely clumped and dispersed
• Multiple basophilic nucleoli
• Necrosis
• Numerous mitotic figures
• Tingible body macrophages ingesting apoptotic debris
• Typically positive for Pan-B-cell markers, CD10, Bcl-6; Mib-1 positive in nearly 100% of cells
• Negative for CD5, Bcl-2, CD23, TdT
• Typically express CD45 (bright), CD19, CD20, CD10, CD38, FMC7; kappa or lambda light-chain restriction
• Negative for CD5
• MYC translocations
• Partners with Ig gene loci: IgH, Ig-kappa, Ig-lambda
• t(8;14)(q24;q32)- 80%
• t(8;22)(q24;q11)- 15%
• t(2;8)(q11;q24)- 5%
• Caraway NP, Stewart JM. Small lymphocytic lymphoma. Diagnostic Pathology Cytopathology. First Edition. DR Mody. Manitoba: Amirsys, 2014. III-4-30-III-4-31. Print.
• Leoncini L, Raphael M, et al. Burkitt lymphoma. WHO Classification of Tumours of Haematopoietic and Lymphoid Tissues. Fourth Edition. Steven H. Swerdlow. Lyon: 2008. 262-264. Print.